1ZP2

Structure of the Mediator subunit cyclin C

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	CCD
Collection date	2003-11-16
Detector	MARRESEARCH
Wavelength(s)	1.0725
Spacegroup name	P 31 2 1
Unit cell lengths	91.700, 91.700, 91.100
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 3.000
R-factor	0.2904
Rwork	0.244
R-free	0.28820
Structure solution method	MAD
RMSD bond length	0.008
RMSD bond angle	1.301
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	3.160
High resolution limit [Å]	3.000	3.000
Number of reflections	9161
Completeness [%]	99.4	99.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	sodium acetate, imidazole, PEG 400, Tris, sodium chloride, glycerol, DTT, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	sodium acetate, imidazole, PEG 400, Tris, sodium chloride, glycerol, DTT, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	sodium acetate, imidazole, PEG 400, Tris, sodium chloride, glycerol, DTT, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K