1ZOV
Crystal Structure of Monomeric Sarcosine Oxidase from Bacillus sp. NS-129
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-10-22 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 171.042, 171.042, 72.455 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.860 |
| R-factor | 0.15937 |
| Rwork | 0.158 |
| R-free | 0.19144 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1L9F |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.338 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.920 |
| High resolution limit [Å] | 1.860 | 1.860 |
| Number of reflections | 101469 | |
| <I/σ(I)> | 27.8 | 3.5 |
| Completeness [%] | 97.8 | 94.6 |
| Redundancy | 5.6 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 0.1M Tris-HCl buffer (pH 8.5), 2.0M ammonium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
