1ZOP
CD11A I-DOMAIN WITH BOUND MAGNESIUM ION
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1994-09 |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 76.160, 78.630, 66.370 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.000 |
| R-factor | 0.197 |
| Rwork | 0.197 |
| R-free | 0.26300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ifa |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.940 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 2.000 |
| Rmerge | 0.107 |
| Number of reflections | 22687 |
| <I/σ(I)> | 20.7 |
| Completeness [%] | 87.1 |
| Redundancy | 5.8 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 5.2 | pH 5.2 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 2 | 1 | drop | 5 (mM) | ||
| 3 | 1 | drop | beta-mercaptoethanol | 5 (mM) | |
| 4 | 1 | reservoir | PEG3350 | 14-20 (%) | |
| 5 | 1 | reservoir | 100 (mM) | ||
| 6 | 1 | reservoir | sodium acetate | 50 (mM) |
