1ZOF
Crystal structure of alkyl hydroperoxide-reductase (AhpC) from Helicobacter Pylori
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-07-17 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.27 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 193.860, 138.420, 127.540 |
| Unit cell angles | 90.00, 125.70, 90.00 |
Refinement procedure
| Resolution | 52.800 - 2.950 |
| R-factor | 0.236 |
| Rwork | 0.236 |
| R-free | 0.26000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1e2y |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.400 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.800 | 3.110 |
| High resolution limit [Å] | 2.950 | 2.950 |
| Rmerge | 0.091 | 0.390 |
| Number of reflections | 57514 | |
| <I/σ(I)> | 7 | 1.6 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 3.1 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4.5 | 293 | sodium acetate, sodium formate, pH 4.5, VAPOR DIFFUSION, temperature 293K |
