1ZOA
Crystal Structure Of A328V Mutant Of The Heme Domain Of P450Bm-3 With N-Palmitoylglycine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-06-24 |
| Detector | SBC-2 |
| Wavelength(s) | 0.94500 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 59.110, 148.050, 63.670 |
| Unit cell angles | 90.00, 98.56, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.740 |
| R-factor | 0.196 |
| Rwork | 0.165 |
| R-free | 0.19600 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1bu7 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.650 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.300 | 1.800 |
| High resolution limit [Å] | 1.740 | 1.740 |
| Rmerge | 0.045 | 0.189 |
| Number of reflections | 110118 | |
| <I/σ(I)> | 27.3 | 6.4 |
| Completeness [%] | 99.6 | 98.2 |
| Redundancy | 4.1 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.3 | 277 | PEG 3350, MES, Magnesium Chloride, Glycerol, pH 6.3, temperature 277K, VAPOR DIFFUSION, HANGING DROP |
