1ZO4
Crystal Structure Of A328S Mutant Of The Heme Domain Of P450BM-3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-04-04 |
| Detector | SBC-2 |
| Wavelength(s) | 1.03320 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.827, 153.209, 61.583 |
| Unit cell angles | 90.00, 94.33, 90.00 |
Refinement procedure
| Resolution | 29.300 - 1.460 |
| R-factor | 0.216 |
| Rwork | 0.189 |
| R-free | 0.21600 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1fag |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.530 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.300 | 1.480 |
| High resolution limit [Å] | 1.460 | 1.460 |
| Rmerge | 0.065 | 0.748 |
| Number of reflections | 177454 | |
| <I/σ(I)> | 20.6 | 1.2 |
| Completeness [%] | 94.1 | 60.8 |
| Redundancy | 4.3 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | PEG 3350, MES, magnesium chloride, Glycerol, pH 6.0, temperature 277K, VAPOR DIFFUSION, HANGING DROP |
