1ZNX
Crystal Structure Of Mycobacterium tuberculosis Guanylate Kinase In Complex With GMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-12-12 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9756 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 112.231, 112.231, 112.231 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.250 - 2.350 |
| R-factor | 0.18302 |
| Rwork | 0.180 |
| R-free | 0.23131 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1znw |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.520 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | CCP4 ((MOLREP)) |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.500 | 2.410 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Number of reflections | 9932 | |
| <I/σ(I)> | 30.7 | 8.9 |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 21.9 | 22.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | 15% (w/v) xylitol, 3.5M sodium chloride, 0.1M bicine, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
