1ZNW
Crystal Structure Of Unliganded Form Of Mycobacterium tuberculosis Guanylate Kinase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-12-13 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9756 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 112.040, 112.040, 112.040 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 18.670 - 2.100 |
| R-factor | 0.19346 |
| Rwork | 0.192 |
| R-free | 0.21976 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1s4q |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.405 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | CCP4 ((MOLREP)) |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 18.670 | 2.154 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 13765 | |
| <I/σ(I)> | 32.7 | 8.2 |
| Completeness [%] | 99.4 | 99.6 |
| Redundancy | 21.8 | 22 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | 15% (w/v) xylitol, 3.5M sodium chloride, 0.1M bicine, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
