1ZNP

X-Ray Crystal Structure of Protein Q8U9W0 from Agrobacterium tumefaciens. Northeast Structural Genomics Consortium Target AtR55.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Collection date	2005-03-15
Detector	ADSC QUANTUM 4
Wavelength(s)	0.97898
Spacegroup name	C 2 2 21
Unit cell lengths	75.967, 91.323, 291.580
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.110 - 2.500
R-factor	0.209
Rwork	0.209
R-free	0.26700
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.600
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.590
High resolution limit [Å]	2.500	2.500
Number of reflections	68197
Completeness [%]	99.9	99.9
Redundancy	8.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1M Na citrate, PEG 20K (20%), VAPOR DIFFUSION, HANGING DROP, temperature 293K