1ZNJ
INSULIN, MONOCLINIC CRYSTAL FORM
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1992-09 |
| Detector | RIGAKU |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.230, 61.650, 48.050 |
| Unit cell angles | 90.00, 110.50, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.000 |
| Rwork | 0.178 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ZINC INSULIN DIMER (PDB ENTRY 4INS) |
| RMSD bond length | 0.019 |
| RMSD bond angle | 0.048 |
| Data reduction software | DENZO |
| Data scaling software | CCP4 |
| Phasing software | CCP4 |
| Refinement software | PROLSQ |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.079 | 0.227 |
| Number of reflections | 22429 | |
| <I/σ(I)> | 9.3 | 3.3 |
| Completeness [%] | 98.6 | 90.1 |
| Redundancy | 3.1 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | batch method | 7.44 | BATCH, 1.7MG/ML INSULIN, 0.02 ZINC ACETATE, 0.7% (V/V) PHENOL, 0.34M SODIUM CHLORIDE, pH 7.44, batch method |
