1ZN6

X-ray Crystal Structure of Protein Q7WLM8 from Bordetella bronchiseptica. Northeast Structural Genomics Consortium Target BoR19.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Collection date	2005-05-06
Detector	ADSC QUANTUM 4
Wavelength(s)	0.97922
Spacegroup name	C 2 2 21
Unit cell lengths	84.406, 87.210, 64.364
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.430 - 1.800
R-factor	0.2033
Rwork	0.203
R-free	0.23000
Structure solution method	SAD
RMSD bond length	0.005
RMSD bond angle	1.300
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.860
High resolution limit [Å]	1.800	1.800
Number of reflections	41842
Completeness [%]	98.4	95.4
Redundancy	7.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	2.4M Ammonium sulfate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K