1ZMX
Crystal structure of D. melanogaster deoxyribonucleoside kinase N64D mutant in complex with thymidine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-09-14 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.939 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 69.712, 70.340, 224.528 |
| Unit cell angles | 90.00, 90.69, 90.00 |
Refinement procedure
| Resolution | 41.170 - 3.100 |
| R-factor | 0.26958 |
| Rwork | 0.269 |
| R-free | 0.28840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ot3 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.134 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.170 | 3.270 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Number of reflections | 39975 | |
| <I/σ(I)> | 3.4 | |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | COUNTER-DIFFUSION | 6.5 | 288 | MES, lithium sulphate, mPEG 2000, pH 6.5, Counter diffusion, temperature 288K |
