1ZMW

Catalytic and ubiqutin-associated domains of MARK2/PAR-1: T208A/S212A inactive double mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X13
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X13
Temperature [K]	100
Detector technology	CCD
Collection date	2004-07-21
Detector	MARRESEARCH
Wavelength(s)	0.8043
Spacegroup name	P 61
Unit cell lengths	119.302, 119.302, 105.694
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	73.880 - 2.802
R-factor	0.20619
Rwork	0.203
R-free	0.26243
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1y8g
RMSD bond length	0.021
RMSD bond angle	1.930
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	73.880	2.850
High resolution limit [Å]	2.802	2.802
Number of reflections	21073
<I/σ(I)>	17.1	2.78
Completeness [%]	99.9	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	PEG 3350, Ammonium Sulphate, BIS-TRIS, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K