1ZMU

Catalytic and ubiqutin-associated domains of MARK2/PAR-1: Wild type

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X13
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X13
Temperature [K]	100
Detector technology	CCD
Collection date	2004-05-08
Detector	MARRESEARCH
Wavelength(s)	0.8046
Spacegroup name	P 61
Unit cell lengths	120.269, 120.269, 99.533
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.140 - 2.900
R-factor	0.19916
Rwork	0.195
R-free	0.27006
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1y8g
RMSD bond length	0.020
RMSD bond angle	2.002
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.140	2.950
High resolution limit [Å]	2.900	2.900
Number of reflections	18222
<I/σ(I)>	27.9	3.3
Completeness [%]	99.9	100
Redundancy	6.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	PEG 3350, Ammonium Sulphate, BIS-TRIS, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K