1ZMQ
Crystal structure of human alpha-defensin-6
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-06-10 |
Detector | MARRESEARCH |
Wavelength(s) | 0.9200 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 62.068, 62.068, 145.297 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 15.000 - 2.100 |
R-factor | 0.17757 |
Rwork | 0.177 |
R-free | 0.19287 |
Structure solution method | SAD |
Starting model (for MR) | Experimental phases |
RMSD bond length | 0.014 |
RMSD bond angle | 1.670 |
Data scaling software | SCALEPACK |
Phasing software | SHELXD |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.180 |
High resolution limit [Å] | 2.100 | 2.100 |
Number of reflections | 17013 | |
<I/σ(I)> | 14.4 | 2 |
Completeness [%] | 97.8 | 93.9 |
Redundancy | 3.6 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | sodium acetate trihydrate, 2-Methyl-2,4-pentanediol, calcium chloride dehydrate, VAPOR DIFFUSION, HANGING DROP, temperature 293K |