1ZMP
Crystal structure of human defensin-5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-01-01 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9200 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 49.375, 49.375, 255.185 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.730 - 1.650 |
| R-factor | 0.20046 |
| Rwork | 0.198 |
| R-free | 0.24027 |
| Structure solution method | SAD |
| Starting model (for MR) | Expermiental phases |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.643 |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Number of reflections | 23239 | |
| <I/σ(I)> | 22.1 | 2.5 |
| Completeness [%] | 90.2 | 50 |
| Redundancy | 8.7 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | HEPES, lithium sulfate monohydrate, dioxane, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
