1ZMM
Crystal structure of human alpha-defensin-4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-09-20 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9200 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 72.004, 39.255, 48.118 |
| Unit cell angles | 90.00, 110.08, 90.00 |
Refinement procedure
| Resolution | 15.000 - 1.600 |
| R-factor | 0.18707 |
| Rwork | 0.185 |
| R-free | 0.21791 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Based on the monomer of HNP-3 (PDB ID code 1DFN) |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.615 |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Number of reflections | 15948 | |
| <I/σ(I)> | 12.1 | 3.5 |
| Completeness [%] | 95.2 | 71.1 |
| Redundancy | 3.3 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | sodium citrate, HEPES, 2-Methyl-2,4-pentanediol, PEG400, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
