1ZMH
Crystal structure of human neutrophil peptide 2, HNP-2 (variant Gly16-> D-Ala)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-06-10 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 42.038, 26.051, 61.587 |
| Unit cell angles | 90.00, 105.79, 90.00 |
Refinement procedure
| Resolution | 19.000 - 1.500 |
| R-factor | 0.17921 |
| Rwork | 0.177 |
| R-free | 0.21252 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | BASED ON MONOMER OF HNP-3 (PDB ENTRY 1DEF) |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.023 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.550 |
| High resolution limit [Å] | 1.480 | 1.480 |
| Rmerge | 0.038 | |
| Number of reflections | 20719 | |
| Completeness [%] | 95.6 | 65.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | lithium sulfate, PEG8000, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
