1ZM2
Structure of ADP-ribosylated eEF2 in complex with catalytic fragment of ETA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-01-13 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.952 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 330.630, 67.840, 191.500 |
| Unit cell angles | 90.00, 103.30, 90.00 |
Refinement procedure
| Resolution | 30.000 - 3.070 |
| R-factor | 0.232 |
| Rwork | 0.231 |
| R-free | 0.26500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | pdb entries 1n0v and 1aer |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.450 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 3.150 |
| High resolution limit [Å] | 3.070 | 3.070 |
| Number of reflections | 75835 | |
| <I/σ(I)> | 9.6 | |
| Completeness [%] | 99.1 | 91 |
| Redundancy | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.2 | 293 | PEG 6000, MPD, HEPES, pH 7.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
