1ZLZ
Re-Evaluation of the Low-Temperature Azide in Mn-Dependent Superoxide Dismutase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-01-13 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.727, 45.676, 95.859 |
| Unit cell angles | 90.00, 97.92, 90.00 |
Refinement procedure
| Resolution | 35.690 - 1.550 |
| R-factor | 0.1817 |
| Rwork | 0.180 |
| R-free | 0.22193 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ix9 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.340 |
| Data reduction software | CrystalClear ((MSC/RIGAKU)) |
| Data scaling software | CCP4 ((TRUNCATE)) |
| Phasing software | MOLREP |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.690 | 1.586 |
| High resolution limit [Å] | 1.550 | 1.546 |
| Rmerge | 0.061 | 0.270 |
| Number of reflections | 52079 | |
| <I/σ(I)> | 13.1 | 2.9 |
| Completeness [%] | 100.0 | 65 |
| Redundancy | 4.02 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 298 | PEG 6000, MPD, TRIS buffer, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
