1ZLP
Petal death protein PSR132 with cysteine-linked glutaraldehyde forming a thiohemiacetal adduct
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-02-21 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.2755, 0.9792, 0.9799, 0.9724 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 156.190, 156.190, 75.050 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 10.000 - 2.700 |
| R-factor | 0.19968 |
| Rwork | 0.195 |
| R-free | 0.24998 |
| Structure solution method | MAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.421 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.500 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.056 | 0.215 |
| Number of reflections | 26327 | |
| <I/σ(I)> | 41.5 | 5.9 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 10.2 | 9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 0.1 M Tris, 12% PEG 20K, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
