1ZLM
Crystal structure of the SH3 domain of human osteoclast stimulating factor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-10-16 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 36.810, 80.480, 49.160 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.070 |
| R-factor | 0.158 |
| Rwork | 0.158 |
| R-free | 0.21160 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gri |
| RMSD bond length | 0.014 |
| RMSD bond angle | 0.030 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.100 | 1.110 |
| High resolution limit [Å] | 1.070 | 1.070 |
| Rmerge | 0.098 | 0.241 |
| Number of reflections | 27403 | |
| <I/σ(I)> | 17.4 | 2.5 |
| Completeness [%] | 86.3 | 49.9 |
| Redundancy | 5.1 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | Sodium Hepes, PEG400, ammonium sulfate, pH 7.5, temperature 277K, VAPOR DIFFUSION, SITTING DROP |
