1ZLM
Crystal structure of the SH3 domain of human osteoclast stimulating factor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-10-16 |
Detector | MARRESEARCH |
Wavelength(s) | 1.0000 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 36.810, 80.480, 49.160 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 1.070 |
R-factor | 0.158 |
Rwork | 0.158 |
R-free | 0.21160 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1gri |
RMSD bond length | 0.014 |
RMSD bond angle | 0.030 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 31.100 | 1.110 |
High resolution limit [Å] | 1.070 | 1.070 |
Rmerge | 0.098 | 0.241 |
Number of reflections | 27403 | |
<I/σ(I)> | 17.4 | 2.5 |
Completeness [%] | 86.3 | 49.9 |
Redundancy | 5.1 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | Sodium Hepes, PEG400, ammonium sulfate, pH 7.5, temperature 277K, VAPOR DIFFUSION, SITTING DROP |