1ZLK
Crystal Structure of the Mycobacterium tuberculosis Hypoxic Response Regulator DosR C-terminal Domain-DNA Complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-03-30 |
| Wavelength(s) | 0.96411 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 142.397, 58.791, 82.933 |
| Unit cell angles | 90.00, 125.50, 90.00 |
Refinement procedure
| Resolution | 50.000 - 3.100 |
| R-factor | 0.27233 |
| Rwork | 0.272 |
| R-free | 0.28802 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zlj |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.086 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.210 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.092 | 0.482 |
| Number of reflections | 8211 | |
| <I/σ(I)> | 10.7 | 2.03 |
| Completeness [%] | 79.5 | 60.7 |
| Redundancy | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 298 | PEG 400, HEPES, calcium chloride, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 400 | ||
| 2 | 1 | 1 | HEPES | ||
| 3 | 1 | 1 | calcium chloride | ||
| 4 | 1 | 1 | H2O | ||
| 5 | 1 | 2 | PEG 400 | ||
| 6 | 1 | 2 | HEPES | ||
| 7 | 1 | 2 | calcium chloride | ||
| 8 | 1 | 2 | H2O |
