1ZL7
Crystal structure of catalytically-active phospholipase A2 with bound calcium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE D03B-MX1 |
| Synchrotron site | LNLS |
| Beamline | D03B-MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-06-20 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.427 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 39.206, 53.218, 90.057 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 22.900 - 1.600 |
| R-factor | 0.17892 |
| Rwork | 0.176 |
| R-free | 0.23199 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1umv |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.722 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.047 | 0.482 |
| Number of reflections | 12748 | |
| Completeness [%] | 99.2 | 98.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | PEG 3350, CaCl2, tris, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
