1ZL0
Structure of Protein of Unknown Function PA5198 from Pseudomonas aeruginosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-03-06 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.678, 79.803, 70.832 |
| Unit cell angles | 90.00, 104.01, 90.00 |
Refinement procedure
| Resolution | 34.500 - 1.100 |
| R-factor | 0.09921 |
| Rwork | 0.099 |
| R-free | 0.12082 |
| Structure solution method | SAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.960 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.500 | 1.120 |
| High resolution limit [Å] | 1.100 | 1.100 |
| Rmerge | 0.028 | 0.301 |
| Number of reflections | 189940 | |
| <I/σ(I)> | 52.3 | 3.38 |
| Completeness [%] | 81.3 | 15 |
| Redundancy | 4.3 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 294 | 0.2 M potassium/sodium tartarate, 20% PEG 3350, pH 7.5, temperature 294K, VAPOR DIFFUSION, HANGING DROP |
