1ZKO

Crystal structure of Glycine cleavage system H protein (tm0212) from Thermotoga maritima at 1.65 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2005-04-04
Detector	ADSC QUANTUM 315
Wavelength(s)	0.946415, 0.979129, 0.979494
Spacegroup name	P 21 21 21
Unit cell lengths	53.950, 65.249, 69.140
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.530 - 1.650
R-factor	0.22724
Rwork	0.225
R-free	0.27000
Structure solution method	MAD
RMSD bond length	0.008
RMSD bond angle	1.065
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	autoSHARP
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	42.530	42.530	1.640
High resolution limit [Å]	1.560	4.930	1.560
Rmerge	0.073	0.036	0.367
Number of reflections	30875
<I/σ(I)>	6.4	11.7	2
Completeness [%]	87.4	93	40
Redundancy	3.6	3.5	1.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION,SITTING DROP,NANODROP	4	277	1.0M LiCl, 30.0% PEG-6000, 0.1M Citrate pH 4.0, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K