1ZKM

Structural Analysis of Escherichia Coli ThiF

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	CCD
Collection date	2004-03-15
Detector	ADSC QUANTUM 315
Wavelength(s)	1.2826, 1.2821, 1.2500
Spacegroup name	F 41 3 2
Unit cell lengths	360.168, 360.168, 360.168
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.950
Rwork	0.229
R-free	0.28000
Structure solution method	MAD
RMSD bond length	0.008
RMSD bond angle	1.434
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.950	2.950
Number of reflections	46923
Completeness [%]	99.5	99.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	Ammonium Acetate, MES, DTT, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	Ammonium Acetate, MES, DTT, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K