1ZKE
1.6 A Crystal Structure of a Protein HP1531 of Unknown Function from Helicobacter pylori
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-12-21 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9797 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 57.156, 73.262, 135.425 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 67.730 - 1.600 |
| R-factor | 0.19996 |
| Rwork | 0.198 |
| R-free | 0.23181 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.199 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.067 | 0.403 |
| Number of reflections | 75865 | |
| <I/σ(I)> | 24.48 | 2.73 |
| Completeness [%] | 92.5 | 67.7 |
| Redundancy | 4.5 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 25% PEG3350, 0.1M NH4SO4, 0.1M Bis-Tris., pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
