1ZKB
Zinc-free Engineered maltose binding protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 105 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-04-01 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 |
| Unit cell lengths | 37.580, 44.010, 57.350 |
| Unit cell angles | 100.25, 101.74, 102.16 |
Refinement procedure
| Resolution | 37.690 - 2.200 |
| R-factor | 0.264 |
| Rwork | 0.244 |
| R-free | 0.28500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1omp |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.048 | 0.124 |
| Number of reflections | 17048 | |
| Completeness [%] | 96.0 | 93.8 |
| Redundancy | 4.7 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | PEG 2000 MME, sodium acetate, Tris-HcL, sodium chloride, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
