1ZK1

Structure of R-specific alcohol dehydrogenase (mutant G37D) from Lactobacillus brevis in complex with phenylethanol and NAD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	ENRAF-NONIUS FR571
Wavelength(s)	1.5418
Spacegroup name	I 2 2 2
Unit cell lengths	55.849, 79.806, 112.622
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	56.300 - 1.780
R-factor	0.14176
Rwork	0.141
R-free	0.16603
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.022
RMSD bond angle	2.085
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall
Low resolution limit [Å]	56.300
High resolution limit [Å]	1.780
Number of reflections	23970

Crystallization Conditions

crystal ID	method	pH	temperature	details
1