1ZJZ

Structure of R-specific alcohol dehydrogenase (mutant G37D) from Lactobacillus brevis in complex with phenylethanol and NAD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Wavelength(s)	0.9155
Spacegroup name	I 2 2 2
Unit cell lengths	55.856, 79.847, 112.915
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.600 - 1.100
R-factor	0.12438
Rwork	0.124
R-free	0.13802
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.016
RMSD bond angle	1.713
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall
Low resolution limit [Å]	35.600
High resolution limit [Å]	1.100
Number of reflections	90750

Crystallization Conditions

crystal ID	method	pH	temperature	details
1