1ZIV
Catalytic Domain of Human Calpain-9
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 32-ID |
| Synchrotron site | APS |
| Beamline | 32-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-04-06 |
| Detector | MARRESEARCH |
| Spacegroup name | P 2 2 21 |
| Unit cell lengths | 45.308, 80.900, 100.545 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.380 - 2.310 |
| R-factor | 0.20684 |
| Rwork | 0.203 |
| R-free | 0.27165 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1mdw |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.413 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.830 | 2.430 |
| High resolution limit [Å] | 2.310 | 2.310 |
| Rmerge | 0.069 | 0.340 |
| Number of reflections | 16773 | |
| <I/σ(I)> | 19.2 | 3.4 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 298 | 20% PEG 3350, 0.2 M Ammonium Formate, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
