1ZIR

Deuterated gammaE crystallin in H2O solvent

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2003-10-10
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9393
Spacegroup name	P 21 21 21
Unit cell lengths	37.530, 43.320, 101.610
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.640 - 1.360
R-factor	0.182
Rwork	0.180
R-free	0.21100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1a5d
RMSD bond length	0.017
RMSD bond angle	1.768
Data reduction software	MOSFLM
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.640	1.450
High resolution limit [Å]	1.360	1.360
Number of reflections	36512
Completeness [%]	99.9	93.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.7	293	PEG 3350, Magnesium acetate, H2O, pH 7.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K