1ZIE

Hydrogenated gammaE crystallin in D2O solvent

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2004-03-11
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9393
Spacegroup name	P 21 21 21
Unit cell lengths	37.630, 43.420, 101.860
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.640 - 1.440
R-factor	0.172
Rwork	0.167
R-free	0.22000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1a5d
RMSD bond length	0.017
RMSD bond angle	1.766
Data reduction software	MOSFLM
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.640	1.530
High resolution limit [Å]	1.440	1.440
Number of reflections	30005
Completeness [%]	97.1	90.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	293	PEG 3350, Magnesium Acetate, D2O, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K