1ZIA

OXIDIZED PSEUDOAZURIN

Experimental procedure

Detector technology	IMAGE PLATE
Collection date	1993-06-25
Detector	RIGAKU RAXIS IIC
Spacegroup name	P 21 21 21
Unit cell lengths	56.690, 61.530, 30.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 1.540
R-factor	0.184 *
Rwork	0.184
RMSD bond length	0.018
RMSD bond angle	0.033
Data reduction software	PROCESS
Refinement software	PROLSQ

Data quality characteristics

	Overall
High resolution limit [Å]	1.540 *
Rmerge	0.042
Total number of observations	28799 *
Number of reflections	10643
Completeness [%]	64.7
Redundancy	2.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	sodium cacodylate	0.1 (M)
3	1	drop	sodium azide	1 (mM)
4	1	drop	ammonium sulfate	50 (%sat)
5	1	reservoir	sodium cacodylate	0.1 (M)
6	1	reservoir	sodium azide	1 (mM)
7	1	reservoir	PEG8000	5 (%(w/v))
8	1	reservoir	ammonium sulfate	70 (%sat)