1ZHL

Crystal structure of HLA-B*3508 presenting 13-mer EBV antigen LPEPLPQGQLTAY

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-C
Synchrotron site	APS
Beamline	14-BM-C
Collection date	2003-11-25
Spacegroup name	P 21 21 21
Unit cell lengths	50.810, 81.340, 110.990
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 1.500
R-factor	0.204
Rwork	0.204
R-free	0.22700
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	46.240	1.560
High resolution limit [Å]	1.500	3.660	1.500
Rmerge	0.062	0.023	0.306
Number of reflections	68535
<I/σ(I)>	20.64	37.6	4.323
Completeness [%]	88.4	93	82.8
Redundancy	2.97

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.6	277	0.2M Ammonium Acetate, 17% w/v PEG 3350, 100mM Cacodylate, pH 7.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K