1ZHJ

Crystal Structure of human N-acetylgalactosaminyltransferase (GTA) Complexed with Galactose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Temperature [K]	113
Detector technology	IMAGE PLATE
Collection date	2004-04-13
Detector	RIGAKU RAXIS IV
Spacegroup name	C 2 2 21
Unit cell lengths	52.700, 149.500, 79.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.590
Rwork	0.198
R-free	0.22000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1lz0
Data reduction software	CrystalClear ((MSC/RIGAKU))
Data scaling software	CrystalClear ((MSC/RIGAKU))
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000
High resolution limit [Å]	1.590	1.590
Rmerge	0.040
Number of reflections	41114
Completeness [%]	96.3	92.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	291	ADA, Sodium Acetate, NaCl, magnesium chloride, MPD, glycerol, PEG 4000, 3-chloro-HG-2-methoxy-propylurea, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K