1ZH7
Structural and Biochemical Basis for Selective Repression of the Orphan Nuclear Receptor LRH-1 by SHP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 32-ID |
| Synchrotron site | APS |
| Beamline | 32-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-08-15 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.99998 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 113.765, 35.126, 134.205 |
| Unit cell angles | 90.00, 92.61, 90.00 |
Refinement procedure
| Resolution | 30.200 - 2.500 |
| R-factor | 0.226 |
| Rwork | 0.226 |
| R-free | 0.28500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.200 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.200 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 21170 | |
| Completeness [%] | 100.0 | 99 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | PEG 3350, tri-Lithium Citrate tetrahydrate, pH 7.0, temperature 293K, VAPOR DIFFUSION, HANGING DROP |
