1ZGS
Parkia platycephala seed lectin in complex with 5-bromo-4-chloro-3-indolyl-a-D-mannose
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM16 |
| Synchrotron site | ESRF |
| Beamline | BM16 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-09-23 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.91995, 0.91932, 0.92191 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 80.239, 114.177, 80.304 |
| Unit cell angles | 90.00, 119.88, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.500 |
| R-factor | 0.2117 |
| Rwork | 0.208 |
| R-free | 0.24387 |
| Structure solution method | MAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 3.160 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0003) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 69.673 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.048 | 0.170 |
| Number of reflections | 43436 | |
| <I/σ(I)> | 15.3 | 2.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 5.8 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 2.0M ammonium dihydrogen phosphate, 0.1M Tris-hydrochloride, 3mM, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
