1ZGR
Crystal structure of the Parkia platycephala seed lectin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM16 |
| Synchrotron site | ESRF |
| Beamline | BM16 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-03-16 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9300 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 63.597, 68.511, 208.531 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.500 |
| R-factor | 0.21525 |
| Rwork | 0.214 |
| R-free | 0.24297 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.711 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0003) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 104.000 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.002 |
| Rmerge | 0.059 | 0.101 |
| Number of reflections | 31543 | |
| <I/σ(I)> | 9.5 | 4.4 |
| Completeness [%] | 100.0 | 65.1 |
| Redundancy | 4.9 | 1.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | PEG 4000, isopropanol, hepes, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
