1ZGQ
Crystal Structure of the Discosoma Red Fluorescent Protein (DsRed) Variant Q66M
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.999 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 74.235, 87.981, 161.639 |
| Unit cell angles | 90.00, 96.72, 90.00 |
Refinement procedure
| Resolution | 37.780 - 1.900 |
| R-factor | 0.194 |
| Rwork | 0.194 |
| R-free | 0.22000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.700 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 157512 | |
| <I/σ(I)> | 10 | 1.8 |
| Completeness [%] | 97.0 | 88.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.2 | 298 | PEG 8000, sodium chloride, phosphate-citrate, pH 4.2, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
