1ZGP
Crystal Structure of the Discosoma Red Fluorescent Protein (DsRed) Variant K70M
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 57.280, 57.228, 71.543 |
| Unit cell angles | 89.74, 100.25, 92.86 |
Refinement procedure
| Resolution | 32.510 - 1.900 |
| R-factor | 0.226 |
| Rwork | 0.226 |
| R-free | 0.26300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.700 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 59952 | |
| <I/σ(I)> | 6 | 2.2 |
| Completeness [%] | 85.7 | 85.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | PEG 4000, magnesium chloride, citrate, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
