1ZG1
NarL complexed to nirB promoter non-palindromic tail-to-tail DNA site
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X8C |
| Synchrotron site | NSLS |
| Beamline | X8C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-03-11 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 76.580, 52.820, 84.970 |
| Unit cell angles | 90.00, 90.07, 90.00 |
Refinement procedure
| Resolution | 9.000 - 2.300 |
| Rwork | 0.257 |
| R-free | 0.28600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1je8 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.300 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.088 | 0.481 |
| Number of reflections | 32539 | |
| <I/σ(I)> | 18.3 | 3.9 |
| Completeness [%] | 98.4 | 98.8 |
| Redundancy | 6.9 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | Ammonium sulfate, pH 7.5, hanging drop, temperature 298K, VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | Ammonium sulfate | ||
| 2 | 1 | 1 | H2O | ||
| 3 | 1 | 2 | Ammonium sulfate | ||
| 4 | 1 | 2 | H2O |
