1ZG1
NarL complexed to nirB promoter non-palindromic tail-to-tail DNA site
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X8C |
Synchrotron site | NSLS |
Beamline | X8C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-03-11 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 76.580, 52.820, 84.970 |
Unit cell angles | 90.00, 90.07, 90.00 |
Refinement procedure
Resolution | 9.000 - 2.300 |
Rwork | 0.257 |
R-free | 0.28600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1je8 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 2.300 |
High resolution limit [Å] | 2.250 | 2.250 |
Rmerge | 0.088 | 0.481 |
Number of reflections | 32539 | |
<I/σ(I)> | 18.3 | 3.9 |
Completeness [%] | 98.4 | 98.8 |
Redundancy | 6.9 | 6.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | Ammonium sulfate, pH 7.5, hanging drop, temperature 298K, VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | Ammonium sulfate | ||
2 | 1 | 1 | H2O | ||
3 | 1 | 2 | Ammonium sulfate | ||
4 | 1 | 2 | H2O |