1ZFG
CTC Duplex B-DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-ID-B |
| Synchrotron site | APS |
| Beamline | 14-ID-B |
| Temperature [K] | 103 |
| Detector technology | CCD |
| Collection date | 2003-06-13 |
| Detector | MARRESEARCH |
| Spacegroup name | P 31 |
| Unit cell lengths | 33.814, 33.814, 47.127 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 16.900 - 1.750 |
| R-factor | 0.314 |
| Rwork | 0.314 |
| R-free | 0.33800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | NDB ENTRY DD0020 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.900 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 16.900 | 1.760 |
| High resolution limit [Å] | 1.710 | 1.710 |
| Rmerge | 0.252 | 0.204 |
| Number of reflections | 5866 | |
| <I/σ(I)> | 3.7 | 3.15 |
| Completeness [%] | 92.3 | 55.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 298 | Na Cacodylate, CaCl2, Spermine, MPD in resevoir, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K, pH 7.00 |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | Na Cacodylate | ||
| 2 | 1 | 1 | CaCl2 | ||
| 3 | 1 | 1 | Spermine | ||
| 4 | 1 | 1 | MPD | ||
| 5 | 1 | 1 | H2O | ||
| 6 | 1 | 2 | Na Cacodylate | ||
| 7 | 1 | 2 | CaCl2 | ||
| 8 | 1 | 2 | MPD | ||
| 9 | 1 | 2 | H2O |
