1ZF3
ATC Four-stranded DNA Holliday Junction
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-D |
| Synchrotron site | APS |
| Beamline | 14-BM-D |
| Temperature [K] | 103 |
| Detector technology | CCD |
| Collection date | 2004-02-26 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 64.950, 24.620, 37.460 |
| Unit cell angles | 90.00, 112.09, 90.00 |
Refinement procedure
| Resolution | 30.080 - 1.840 |
| R-factor | 0.248 |
| Rwork | 0.202 |
| R-free | 0.23100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | ndb entry UD0058 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.663 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.000 | 1.920 |
| High resolution limit [Å] | 1.840 | 1.840 |
| Rmerge | 0.061 | 0.626 |
| Number of reflections | 4856 | |
| <I/σ(I)> | 15 | 1.07 |
| Completeness [%] | 98.6 | 96.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | Na Cacodylate, CaCl2, Spermine, MPD in resevoir, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | Na Cacodylate | ||
| 2 | 1 | 1 | CaCl2 | ||
| 3 | 1 | 1 | Spermine | ||
| 4 | 1 | 1 | MPD | ||
| 5 | 1 | 1 | H2O | ||
| 6 | 1 | 2 | Na Cacodylate | ||
| 7 | 1 | 2 | CaCl2 | ||
| 8 | 1 | 2 | MPD | ||
| 9 | 1 | 2 | H2O |
