1ZEV
Crystal Structure of a Pathogenic RNA: CUG Repeats
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-07-25 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | H 3 |
| Unit cell lengths | 39.102, 39.102, 141.277 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.550 - 1.580 |
| R-factor | 0.224 |
| Rwork | 0.218 |
| R-free | 0.27900 |
| Structure solution method | MAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 2.060 |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.550 | 1.620 |
| High resolution limit [Å] | 1.580 | 1.580 |
| Number of reflections | 11050 | |
| <I/σ(I)> | 40.5 | 5.1 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 6.2 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | MOPS, NaCl, MgCL2, MPD, pH 7.00, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | MOPS, NaCl, MgCL2, MPD, pH 7.00, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | MOPS, NaCl, MgCL2, MPD, pH 7.00, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MOPS | ||
| 2 | 1 | 1 | NaCl | ||
| 3 | 1 | 1 | MgCL2 | ||
| 4 | 1 | 1 | MPD | ||
| 5 | 1 | 1 | H2O | ||
| 6 | 1 | 2 | NaCl | ||
| 7 | 1 | 2 | MgCL2 | ||
| 8 | 1 | 2 | MPD | ||
| 9 | 1 | 2 | H2O |
