1ZES
BeF3- activated PhoB receiver domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-02-26 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97176 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 77.869, 77.869, 109.071 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.700 - 1.900 |
| R-factor | 0.19365 |
| Rwork | 0.189 |
| R-free | 0.24006 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.567 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 25424 | |
| <I/σ(I)> | 18.43 | 1.71 |
| Completeness [%] | 93.7 | 86.4 |
| Redundancy | 11.71 | 7.89 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.9 | 298 | sodium thiocyanate, Peg3350, pH 6.9, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
