1ZEF
structure of alkaline phosphatase from human placenta in complex with its uncompetitive inhibitor L-Phe
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-06-06 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.98 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 87.791, 114.944, 106.341 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 15.000 - 1.900 |
R-factor | 0.12861 |
Rwork | 0.126 |
R-free | 0.17322 |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.017 |
RMSD bond angle | 1.720 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | FFT |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 99.000 | 1.930 |
High resolution limit [Å] | 1.890 | 1.890 |
Number of reflections | 43024 | |
<I/σ(I)> | 4.6 | |
Completeness [%] | 98.5 | 98.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 295 | PEG, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 295K |