1ZDW
Co-crystal structure of Orf2 an aromatic prenyl transferase from Streptomyces sp. strain CL190 complexed with GSPP and Flaviolin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-05-21 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.82653 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 73.563, 91.609, 48.589 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.590 - 2.020 |
| R-factor | 0.225 |
| Rwork | 0.225 |
| R-free | 0.26400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.100 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.090 |
| High resolution limit [Å] | 2.020 | 2.020 |
| Number of reflections | 21790 | |
| <I/σ(I)> | 31.9 | 38 |
| Completeness [%] | 99.1 | 99.5 |
| Redundancy | 4.5 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 8.5 | 279 | 28% PEG 4000, 0.3M Magnesium Nitrate, 2mM DTT, 0.1M PIPES, pH 8.5, EVAPORATION, temperature 279K |
