1ZDL

Crystal Structure of Mouse Thioredoxin Reductase Type 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2005-02-26
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	I 41 2 2
Unit cell lengths	109.070, 109.070, 204.670
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.690 - 3.000
R-factor	0.241
Rwork	0.241
R-free	0.31000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1h6v
RMSD bond length	0.008
RMSD bond angle	1.500
Data scaling software	d*TREK (9.2SSI)
Phasing software	CNS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.680	3.110
High resolution limit [Å]	3.000	3.000
Rmerge	0.114	0.431
Number of reflections	12754
<I/σ(I)>	17.8	6.6
Completeness [%]	100.0	100
Redundancy	16.29	16.66

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	291	100 mM MES, 24% Peg 550 MME, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K